Fumonisin b2 production by aspergillus niger in thai coffee beans. Request pdf fumonisin b2 production by aspergillus niger from grapes and natural occurrence in must aspergillus niger has been recently found to produce fumonisin b2 fb2. Production of fumonisin b2 and b4 by aspergillus niger on. Fumonisin b 1 is the most prevalent member of a family of toxins, known as fumonisins, produced by several species of fusarium molds, such as fusarium verticillioides, which occur mainly in maize corn, wheat and other cereals. With fumonisin, the highest levels were seen with 20% moisture, and production as well as fungal growth ceased at less than 18%. Diversity of black aspergilli and mycotoxin risks in grape. Fumonisin b2 production by aspergillus niger from thai coffee. Fumonisin b2 production by aspergillus niger request pdf. Read removal of fumonisin b1 and b2 from model solutions and red wine using polymeric substances, food chemistry on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. Aspergillus niger fumonisin b2 ochratoxin a fumonisin grapes wine.
Aspergillus niger is a member of a group of species named aspergillus section nigri, formerly known as a. This study evaluated 681 strains of black aspergilli species isolated from portuguese wine grapes for fb2 production when grown on czapek yeast agar. Review article pathogenecity of aspergillus niger in plants. Paramee noonim, warapa mahakarnchanakulb, kristian f nielsen, jens frisvad, robert a samson. The toxin can be found in commodities such as maize, and in dried figs, and some plants didwania and joshi, 20.
The interaction between the two filamentous model microbes streptomyces coelicolor a32 m145 and aspergillus niger n402 was studied in co. Mycotoxin risk in the grape product chain is primarily due to ochratoxin a ota occurrence in wine and dried vine fruits. There are a total of 30 strains including 27 strains of aspergillus niger intended used in chinese food industry, two strains used as control and one strain isolated from corn for fumonisin fb production on 3 media. The aim of this study was to assess the presence of aspergillus section nigri in brazil nut samples at different stages of its production chain and to verify the toxigenic potential for fumonisin b2 fb2 production of these isolates along with the. Concerns about their safety have arisen with the discovery that some isolates of both species produce fumonisin fb and ochratoxin a ota mycotoxins. Thrane2007fumonisin b 2 production by aspergillus niger. Mansson m, klejnstrup ml, phipps rk, nielsen kf, frisvad jc, et al. Of the media tested, only rice corn steep agar supported fumonisin production by both f. Aspergillus niger has been isolated from 37 genera of plants farr et al. Pdf fumonisin b 2 production by aspergillus niger jorn. Genetic engineering is reported as a promising means of detoxifying mycotoxins. Lyngby, denmark b centraalbureau voor schimmelcultures, p.
This made it possible to develop a predictive model which provided a good linear regression fit between the predicted and observed fumonisin production. Fb2 was not detected in aspergillus carbonarius n 75 or. No ochratoxins were detected in the collected samples, while fumonisin b2 was detected in 37. During 2006 and 2007, a total of 64 thai dried coffee bean samples coffea arabica from two growing sites in chiangmai province and 32 thai dried coffee bean samples coffea canephora from two growing sites in chumporn province, thailand, were collected and assessed for fumonisin contamination by black aspergilli. The dominant species include fusarium verticillioides, f. Corn pasta the production of a simple corn pasta resulted in a decrease in. In contrast, this fumonisin production was inversely related to fum14 signal. Variation in fumonisin and ochratoxin production associated with differences in biosynthetic gene content in aspergillus niger and a. In vitro control of growth and fumonisin production by fusarium verticillioides and. Aspergillus niger is perhaps the most important fungus used in biotechnology, and is also one of the most commonly encountered fungi contaminating foods and feedstuffs, and occurring in soil and indoor environments. When grapederived products were subsequently analyzed, fb2 contamination was found in raisins, must, and wine. Fumonisin b2, known from fusarium verticillioides and other fusaria, was detected in cultures of three full genome sequenced strains of a. Dynamic fumonisin b2 production by aspergillus niger intented.
Proteome analysis of aspergillus niger staff of the. Many strains of aspergillus niger growing naturally on grapes are capable of producing fumonisin b 2, so it is now a concern for human consumption of grapes, raisins, and wine. Characterization of four new antifungal yanuthones from. Fumonisin b 2 fb 2 represents an additional potential mycotoxin risk in the grape.
Incidence of fumonisin b2 production by aspergillus niger. Fumonisin b 2 fb 2 from fusarium moniliforme has been used. Molecular characterization of black aspergillus species from onion and their potential for ochratoxin a and fumonisin b2 production. Aspergillus niger is one of the fungi that has been labelled with the gras. Integrating toxin gene expression, growth and fumonisin b1. Many of its industrial applications have been given gras status generally regarded as safe. Fumonisin b2 production by aspergillus niger journal of.
Fumonisin b2, known from fusarium verticillioides and other fusaria, was. The black fungus aspergillus niger is a significant contaminant of food and feeds, and a widely used cell factory for production of citric acid and bulk enzymes. Fb1 fumonisin b1 fb2 fumonisin b2 fb 3 fumonisin b 3 h2o water hplc high performance liquid chromatography meoh methanol sax strong anion exchange. Aspergillus niger is a filamentous fungus found in the environment, on foods andfeeds and is used as host for production of organic acids, enzymes and proteins. Inhibits sphinganine nacyltransferase ceramide synthase at ic 50 0. Dynamic fumonisin b2 production by aspergillus niger. Fumonisin b2 production by aspergillus niger from thai coffee beans. This is the first record of freshly isolated aspergillus niger strains producing fumonisins. Nov 19, 2010 the potential risk of contamination by fumonisin b2 fb2, although at low levels, has been demonstrated in must and wine. Bertholletia excelsa is the tree that produces brazil nuts which have vast economic importance in the amazon region and as an export commodity. Mycotoxin, structurally similar to fumonisin b 1 prod. Fumonisin b2 fb2 was recently found to be produced by aspergillus niger. C03061 investigation of the loss of parent fumonisin. Focus was on industrial strains, but for completeness strains from the remaining 17 related black aspergillus species were included.
Frisvada a center for microbial biotechnology, department of systems biology, technical university of denmark, soltofts plads 221, dk2800 kgs. Apr 30, 2015 the ochratoxin a and fumonisin b2 contents of the onion samples were examined. Fumonisin measurement from maize samples by highperformance liquid chromatography coupled with corona charged aerosol detector. Application fumonisin b 2 fb 2 from fusarium moniliforme has been used. Pdf fumonisin b2 production by aspergillus niger in thai. Aspergillus acidus from puerh tea and black tea does not. Aspergillus niger is one of the most commonly reported fungi recovered from foods, responsible for the post harvest decay of fresh fruits, it is commonly extracted from nuts, cereals, pulses and oilseeds. Eighteen of 37 isolates of aspergillus welwitschiae were recognized as potential producers for fumonisin b2.
Fumonisin b1 contamination of maize has been reported worldwide at mgkg levels. Aspergillus acidus from puerh tea and black tea does not produce ochratoxin a and fumonisin b 2 j. Fumonisin and ochratoxin production in industrial aspergillus. Incidence of fumonisin b2 production by aspergillus niger in portuguese wine regions. In vitro control of growth and fumonisin production by. During 2006 and 2007, a total of 64 thai dried coffee bean samples coffea arabica from two growing sites in chiangmai province and 32 thai dried coffee bean samples coffea canephora from two growing sites in chumporn province, thailand, were. Lactate added in starchcontaining medium can increase production of the mycotoxin fumonisin b2 by modifying acetylcoa metabolism. This disease is a limiting factor for vegetable production effecting both their quality and quantity larkin and fravel. Fumonisin b2 inhibits sphingosine acyltransferase fumonisin b2 and other fumonisins frequently contaminate maize and other crops references. Fumonisin b2 production by aspergillus niger in thai coffee.
Fumonisin b2 and other fumonisins frequently contaminate maize and other crops. No fusarium species known to produce fumonisin were detected, but black. The potential risk of contamination by fumonisin b2 fb2, although at low levels, has been demonstrated in must and wine. Black aspergilli in general and aspergillus niger in particular are considered to be the major responsible agents of fb2 contamination in grape and its byproducts. Fumonisin b2 sphingosinenacyltransferaseinhibitor 116355. Fumonisin b2 is a fumonisin mycotoxin produced by the fungi fusarium verticillioides and fusarium moniliforme. Molecular characterization of black aspergillus species from.
Fumonisin b2 production by aspergillus niger from thai. Fumonisin b1 contamination of maize has been reported worldwide at. The recent discovery of fumonisin production in aspergillus niger, raises concerns about the presence of these mycotoxins in grapes and raisins as well as other commodities where a. Fumonisin b2 production by aspergillus niger from thai coffee beans paramee noonim, warapa mahakarnchanakulb, kristian f nielsen, jens frisvad, robert a samson to cite this version.
There are reports of aspergillus niger being a plant pathogen in peanuts jackson, 1962. Apparently, aspergillus niger can induce a crown rot of peanuts due to aspergillus niger infected seed under specific hot, humid growth conditions. The carcinogenic mycotoxin fumonisin b2 was detected for the first time in the industrially important aspergillus niger. Variation in fumonisin and ochratoxin production associated. Here we investigate the potential production of fumonisins in a. Fumonisin b2 production by aspergillus niger in thai.
A general inhibition effect was observed with tested. Review article pathogenecity of aspergillus niger in. The fumonisin mycotoxins of which fumonisin group b is the most prevalent and notable one with fumonisin b1 fb1 and fumonisin b2 fb2 being esterified with tricarboxylic and polyhydric alcohols martins et al. Aspergillus niger is one of the most important industrial filamentous fungal species used in biotechnology where it is used extensively for organic acid production and for the production of extracellular enzymes.
This model is important for developing an integrated systems approach by combining gene expression, ecophysiological influences and growth data to predict fumonisin production. Jun 01, 2017 read removal of fumonisin b1 and b2 from model solutions and red wine using polymeric substances, food chemistry on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. Aspergillus niger is a phytopathogenic fungus responsible for the plant disease. Incidence of fumonisin b2 production by aspergillus niger in. Aspergillus niger is also reported to produce ochratoxin a and fumonisin b2 and aflatoxins abraca et al. Nov 14, 2007 the carcinogenic mycotoxin fumonisin b2 was detected for the first time in the industrially important aspergillus niger. Fumonisin b2 production by aspergillus niger from grapes. Extrolite production other than fumonisin b 2 by isolates of aspergillus niger from thai green coffee beans. This research investigate the biological control of ochratoxin a ota production by. Molecular characterization of black aspergillus species. Reports on the ability of aspergillus niger species to produce fumonisins have continued to appear. Samson a 6 7 8 a cbs fungal biodiversity centre, uppsalalaan 8, 3584 ct utrecht, the netherlands 9 b department of food science and technology, faculty of agroindustry, kasetsart university. The mycotoxin fumonisin b2 was recently found to be produced by a. This approach may provide innovative solutions to the problem of fumonisin in corn.